Impact of siRNA-mediated down-regulation of CD147 on human breast cancer cells / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the influence of siRNA-mediated down-regulation of CD147 on growth, proliferation and movement of human breast cancer cell line MDA-MB-231.</p><p><b>METHODS</b>The protein expression of CD147, MMP-2 and TIMP-2 of the MDA-MB-231 cells were analyzed by ABC. Lentiviral expression vector of CD147 gene was constructed and transfected into MDA-MB-231 cells. RT-PCR and Western blot were used to detect the mRNA and protein level changes of CD147 genes to identify the optimal time point, followed by detection of changes of mRNA and protein expression of MMP-2 and TIMP-2 genes. CCK-8 reagent method and cell scratch test were used to detect the proliferation and migration change of MDA-MB-231 cells. The nude mouse model of breast cancer by hypodermic injection with MDA-MB-231 cells was established to document the effect of CD147 siRNA on the tumor transplants.</p><p><b>RESULTS</b>After transfection of lentiviral expression vector of CD147 gene, protein of CD147, MMP-2 and TIMP-2 were weakly or negative expressed, significantly weaker than those of control group (P < 0.01). After 72 hours of transfection, average down-regulation rate of CD147 and MMP-2 were 96.03% ± 0.84% and 96.03% ± 0.84%, respectively. Both CD147 mRNA and MMP-2 mRNA expression were down-regulated (P < 0.05), while TIMP-2 mRNA expression showed no significant deference (P > 0.05). No less than 2 days after transfection, cell growth of MDA-MB-231 cell line was found significantly inhibited (P < 0.05). After 24 hours of transection, average migration distance of MDA-MB-231 cell line and control group were (0.64 ± 0.12) mm and (4.69 ± 0.85) mm, respectively, which indicated a lower migrate speed. Down regulation of CD147 led to reduction of volume and mass of nude mouses. The growth of the carcinoma transplant was inhibited upon siRNA-mediated down-regulation of CD147 (P < 0.05), with an average tumor mass of (1.85 ± 0.98) g and both reduction of tumor size and tumor mass.</p><p><b>CONCLUSIONS</b>CD147 may alter the MMP-2/TIMP-2 balance in MDA-MB-231 cells. CD147 gene silencing inhibits the proliferation and migration of MDA-MB-231 cells and the growth of carcinoma transplants in nude mice.</p>

Effects of CD147 gene silencing on protein expression of ANXA2, MMP-2 and TIMP-2 by thyroid medullary carcinoma TT cells and biologic characteristics / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the influence of CD147 gene silencing on the expression of ANXA2, MMP-2 and TIMP-2 of thyroid medullary carcinoma TT cells and related biological characteristics.</p><p><b>METHODS</b>Protein expression of CD147, ANXA2, MMP-2 and TIMP-2 was detected by immunocytochemistry.RNAi technology was used to identify the specific siRNA sequences and the optimal time point of effective inhibition of CD147 gene. The expression of ANXA2, MMP-2 and TIMP-2 at mRNA and protein levels was detected with RT-PCR and Western blot, respectively. MTT method was used to detect the proliferation of the TT cells, flow cytometry (FCM) to detect the cell cycle and apoptosis changes of TT cells and transwell chamber assays to document the influence of CD147 gene silencing on migration and invasion of the TT cells.</p><p><b>RESULTS</b>The protein expression of CD147, ANXA2, MMP-2 and TIMP-2 proteins was variable in the TT cells. Two siRNA sequences were identified to effectively silence CD147 gene in the TT cells, in which relative expression of MMP-2 was reduced at both mRNA and protein levels; although the expression of ANXA2 mRNA and protein did not change significantly. TIMP-2 protein expression markedly decreased in an absence of its mRNA expression. The proliferation of the TT cells was inhibited upon the CD147 gene silencing along with a significant increase of G(0)/G(1) phase cells and a decrease of G(2)/M phase cells.However, the proportion of the apoptotic cells in all experimental groups did not change. The number of the penetrating cells through the membrane filters did not show significant changes in all experimental groups in the Transwell chamber assays.</p><p><b>CONCLUSIONS</b>Through RNAi technology, two CD147 siRNA sequences are identified and shown to effectively inhibit CD147 gene expression of the TT cells. CD147 gene silencing leads to growth inhibition of the TT cells and alteration of the cell cycle. However, silencing CD147 does not significantly affect the apoptosis, migration and invasion of the TT cells.</p>

Macro-micro-anatomy of the Arterial Network around the wrist Joint / 第三军医大学学报

Fifty prepared specimens of adult upper extrimity were employed in the study,The arteries of each extremity were perfused with red latex and the dissection was carried out under an operating microscope.Twelve arteries participate in the formation of the arterial network around the wrist joint.It consists of 5 divisions situated in different tissue layers,3 on the dorsal and 2 on the palmar side of the extremity.The 2 divisions situated on the dorsal and palmar side of the joint capsule of the wrist respectively are the most completely constructed among the 5,and the main routes for the collateral circulation around the wrist.The dorsal division of the carpal arterial network is mainly formed by the dorsal carpal branch of the radial artery,the dorsal branch of the,anterior in- terosseus artery,and the ascending branch of the superior perforating artery,while the palmar division by the palmar carpal branch of the radial artery,the palmar branch of the anterior interosseus artery,and the dorsal carpal branch of the ulnar artery.There are extensive and multiple anastomoses between the anterior interosseus artery and the radial and ulnar arteries with a preponderance of the anastomoses over the radial side of the extremity in regard to the size and number of the participating vessels. The collateral circulation established by the anastomoses between the dorsal and palmar divisions in preponderant over the dorsal side of the extremity.The carpal arterial network is characterized by its multiple arterial origins,multiple anastomoses and multiple divisions to conform to the poly-articular construction and multi-directional movement of the wrist joint.In addition,the compensation of the blood circulation of the hand through the carpal arterial network in ease the radial artery and/or the ulnar artery are severed or gradually obliterated was discussed morphologically.